AbSorber

XM-ONE® - Troubleshooting

Please observe that the flowchart below is followed by explanatory text

Step 1
Important: Do not to use EDTA tubes.

Step 2
No obvious handling errors.

Step 3
Step 7 in Instructions for use.

Potential problem:	Low yield of endothelial precursor cells.
Potential cause:	Cells are adhering to the tube.
Solution:	Use siliconized tubes according to the instructions.

Step 8 in Instructions for use.

Potential problem:	Low yield of endothelial precursor cells.
Potential cause:	Poor mixing during incubation with magnetic beads or too intense mixing.
Solution:	Ensure that mixing is sufficient to avoid sedimentation but not so intense that the beads fail to bind to the cells.

Step 4
Step 10 in Instructions to use.

Potential problem:	False-positive XM-One.
Potential cause:	PBS not properly discarded before serum added.
Solution:	Make sure that all PBS is discarded according to the Instructions for use.

Step 5
No obvious handling errors.

Step 6

Potential problem:	1. Incorrect setting in flow cytometry, often of the forward-scatter (FSC) and side-scatter (SSC). It is important to adjust the settings so that distinct cell populations can be detected. 2. Wrong cell population obtained.
Potential cause:	Poor mixing during incubation with magnetic beads or too intense mixing.
Solution:	Experience and handling-knowledge of flow cytometry. Different equipment types require different settings. Settings used for lymphocyte crossmatches can often be used with only minor adjustments.